Confocal Facility Instruments
Zeiss LSM-510 META confocal microscope
Location: Room 6605, Cancer Research Building
Phone number: 314-362-2526
Reference: LSM
510
Features
- Argon (458, 477, 488, 514 nm), Helium-Neon (He-Ne)
1 (543 nm) and He-Ne 2 (633 nm) lasers for fluorescence
excitation
- 2 PMT channels and META detector
- META detector for 8 channel collection and spectral
analysis
- Zeiss Axiovert 200M (inverted) microscope for
fixed or live cell work
- Transmitted light detector for DIC images
- 10x, 20x, 40x and 63x oil objectives
- Zeiss LSM-510 operating software, and 3D image
processing
- FRAT, FRAP and Z-stack imaging capabilities
- Coverslip dish holds cells grown on 25 mm glass
coverslips
Bio-Rad MRC 1024 confocal microscope
Room 6613, Cancer Research Building
Phone number: 314-362-5299
Reference: Zeiss
Cell Science
Features
- Krypton-argon (488, 568 and 647 nm) Laser for
fluorescence excitation
- 3 PMT channels for simultaneous and sequential
fluorescence detection
- Upright Zeiss microscope for fixed and live cell
work
- 10x, 20x, 40x and 63x oil objectives, water immersion
objective possible
- Lasersharp operating software
- 3-D image processing Z-stack imaging
Stallion fluorescence imaging microscope
Room 4912, South Building
Phone number: 314-362-5299
References : SlideBook
Software, Camera
Features
- 175 W HBO light source for fluorescence excitation
(from UV to infer red)
- Fluorescence excitation ratioing imaging, such
as fura-2 for [Ca2+]
- Digital camera, cooled CCD CASCADE 512B Able to
acquire >100 images/sec frame rate
- Zeiss Axiovert 200M (inverted) microscope for
fixed or live cell work
- Transmitted light detector for DIC images
- 10x, 20x, 40x and 63x, 100x oil objectives
- SlideBook operating software, 3-D imaging processing,
and 3 mathematical algorithms for deconvolution
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